Chinese Journal Of Cell And Stem Cell >Archive >2018 >Volume 8 Number 6 Dec 2018 >Original Researches >contents

Journal content

Engineer a microenvironment for islet transplantation with bone marrow mesenchymal stroma cells and PDMS scaffold

Chen Jin, Fu Yunfeng, Zhao Hongzhou, et al


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【Abstract】 Objective The study was aimed to engineer a suitable microenvironment for islets survival. Methods The three-dimensional macroporous scaffold was constructed with polydimethylsiloxane (PDMS) and sodium chloride crystal. The mini "artificial pancreas" was engineered with PDMS scaffold, bone marrow mesenchymal stroma cells (MSCs), fibronectin and islets. The artificial pancreases were transplanted into the omentum of STZ-induced diabetic rats, while the control group underwent sham operation. The blood glucose level of the rats was monitored every other day; the data was analyzed by t test and Kolmogorov-Smirnov test/Mann-Whitney test. Results The PDMS scaffolds were constructed successfully with a large number of macro-pores. Islets and MSCs can be loaded into the scaffolds. HE staining showed that there were islets in the scaffold pores with surrounding MSCs. At each time point after transplantation (1, 3, 5, 7 days), the blood glucose levels of diabetic rats in artificial pancreas transplantation group were (278.70±86.06) mg/dl; (323.50±44.29) mg/dl; (283.30±74.0) mg/dl; and (304.80±13.33) mg/ dl respectively, which were significantly decreased than those in the control respectively group (606.00±52.40) mg/dl, (589.70±55.78) mg/dl, (615.00±54.84) mg/dl, (630.30±48.17) mg/ dl, (t = 7.96、9.15、8.82, U = 0.00, P < 0.01). Conclusion The microenvironment engineered with bone marrow MSCs and PDMS scaffold could provide a suitable environment for transplanted islets and provide a new strategy for clinical islet transplantation.
【Key words】 Mesenchymal stroma cells; PDMS; Islet transplantation; Diabetes