Chinese Journal Of Cell And Stem Cell >Archive >2020 >Volume 10 Number 3 June 2020 >Original Researches >contents

Journal content

Effect and mechanisms of miR-98-5p targeting PYGO2 gene on proliferation, migration and invasion of cervical cancer

ShouQuan, Wang Qian, Tan Zhiyuan


[138]

【Abstract】 Objective To investigate the effects and mechanisms of miR-98-5p on the proliferation, migration and invasion of cervical cancer cells. Methods Cancer tissues and adjacent tissues of cervical cancer patients admitted to the first people's Hospital of Chenzhou City from January 2016 to January 2019 were collected; miR-98-5p, si-PYGO2 and anti-miR-98-5p were individually or co-cultured in Siha cells, and named miR-NC group, miR-98-5p group, and si-NC Group, si-PYGO2 group, anti-miR-NC group, anti-miR-98-5p group, miR-98-5p+pcDNA group, miR-98-5p+pcDNA-PYGO2 group. qRT-PCR was used to detect the expressions of miR-98-5p and PYGO2 mRNA in cervical cancer tissues and cells; Western blot was used to detect protein expression; MTT method to detect cell proliferation activity; Transwell to detect cell migration and invasion. WT-PYGO2 and MUT-PYGO2 were co-transfected with miR-NC and miR-98-5p into Siha cells, and the fluorescent activity was detected by dual luciferase reporter gene detection experiment. Statistical analysis was performed using method analysis and t-test. Results Compared with adjacent tissues, the expression of miR-98-5p in cervical cancer tissue was significantly reduced (0.98±0.08 vs 0.47±0.05), PYGO2 mRNA (1.00±0.07 vs 2.43±0.24) and protein expression (0.27±0.03 vs 0.62±0.05) was increased (all P < 0.001). Compared with normal cervical cells Ect1/E6E7, PYGO2 mRNA (0.98±0.09 vs 2.76±0.23, 2.46±0.24, 2.55±0.21)and protein levels (0.21±0.03 vs 0.62±0.06, 0.51±0.05, 0.57±0.06)in cervical cancer cells Siha, Hela, and Caski was increased; the expression of miR-98-5p (1.00±0.08 vs 0.34±0.04, 0.56±0.05, 0.46±0.04)was decreased (all P < 0.05). Compared with the miR-NC group, the activity of cervical cancer Siha cells in the miR-98- 5p group (48 h: 0.42±0.04 vs 0.61±0.05, 72 h: 0.59±0.06 vs 1.02±0.09), the number of migration (48.35±4.96 vs 112.46±10.27)and invasions (39.46±3.52 vs 92.47±9.56) were decreased(all P < 0.05). Compared with the si-NC group, the activity of cervical cancer Siha cells in the si-PYGO2 group (48 h: 0.46±0.05 vs 0.64±0.06, 72 h: 0.67±0.06 vs 1.05±0.08), the number of migration (42.16±4.25 vs 106.48±9.75) and  invasions cells (35.42±6.20 vs 87.63±8.11) were decreased (all P < 0.05); the expressions of Cyclin D1, MMP-2, MMP-9, MMP-14 proteins were decreased, the expressions of p21 and p27 proteins were increased, and the difference was statistically significant(all P < 0.05). Compared with the miR-NC group, the luciferase activity (0.38±0.04 vs 0.99±0.08) was decreased of Siha cells transfected with WT-PYGO2 in miR-98- 5p group (P < 0.05), and the fluorescence of Siha cells transfected with MUT-PYGO2 (1.03±0.08 vs 1.01±0.09) was not significantly different (P > 0.05). Overexpression of PYGO2 reversed the inhibitory effect of miR-98-5p overexpression on the proliferation, migration and invasion of cervical cancer Siha cells. Conclusion miR-98-5p could inhibit the proliferation, migration and invasion of cervical cancer cells, which may be related to its targeted regulation of PYGO2 expression, which will provide a new target for the prevention and treatment of cervical cancer.
 

【Key words】 MiR-98-5p; PYGO2; Cervical cancer; Proliferation; Migration; Invasio